Resistance gene cloning from a wild crop relative by sequence capture and association genetics

Sanu Arora; Burkhard Steuernagel; Kumar Gaurav; Sutha Chandramohan; Yunming Long; Oadi Matny; Ryan Johnson; Jacob Enk; Sambasivam Periyannan; Narinder Singh; M Asyraf Md Hatta; Naveenkumar Athiyannan; Jitender Cheema; Guotai Yu; Ngonidzashe Kangara; Sreya Ghosh; Les J Szabo; Jesse Poland; Harbans Bariana; Jonathan D G Jones; Alison R Bentley; Mick Ayliffe; Eric Olson; Steven S Xu; Brian J Steffenson; Evans Lagudah; Brande B H Wulff

doi:10.1038/s41587-018-0007-9

Resistance gene cloning from a wild crop relative by sequence capture and association genetics

Nat Biotechnol. 2019 Feb;37(2):139-143. doi: 10.1038/s41587-018-0007-9. Epub 2019 Feb 4.

Authors

Sanu Arora¹, Burkhard Steuernagel¹, Kumar Gaurav¹, Sutha Chandramohan², Yunming Long³, Oadi Matny⁴, Ryan Johnson⁴, Jacob Enk⁵, Sambasivam Periyannan², Narinder Singh⁶, M Asyraf Md Hatta^{1

7}, Naveenkumar Athiyannan^{2

8}, Jitender Cheema¹, Guotai Yu¹, Ngonidzashe Kangara¹, Sreya Ghosh¹, Les J Szabo⁹, Jesse Poland⁶, Harbans Bariana¹⁰, Jonathan D G Jones¹¹, Alison R Bentley¹², Mick Ayliffe², Eric Olson¹³, Steven S Xu¹⁴, Brian J Steffenson⁴, Evans Lagudah², Brande B H Wulff¹⁵

Affiliations

¹ John Innes Centre, Norwich Research Park, Norwich, UK.
² Commonwealth Scientific and Industrial Research Organization Agriculture and Food, Canberra, Australian Capital Territory, Australia.
³ Department of Plant Sciences, North Dakota State University, Fargo, ND, USA.
⁴ Department of Plant Pathology, University of Minnesota, St. Paul, MN, USA.
⁵ Arbor Biosciences, Ann Arbor, MI, USA.
⁶ Wheat Genetics Resource Center, Department of Plant Pathology, Kansas State University, Manhattan, KS, USA.
⁷ Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Malaysia.
⁸ Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Brisbane, Queensland, Australia.
⁹ US Department of Agriculture, Agriculture Research Service, Cereal Disease Laboratory, St. Paul, MN, USA.
¹⁰ The University of Sydney Plant Breeding Institute, Cobbitty, New South Wales, Australia.
¹¹ The Sainsbury Laboratory, Norwich Research Park, Norwich, UK.
¹² The John Bingham Laboratory, NIAB, Cambridge, UK.
¹³ Michigan State University, East Lansing, MI, USA.
¹⁴ US Department of Agriculture, Agriculture Research Service, Northern Crop Science Laboratory, Cereal Crops Research Unit, Red River Valley Agricultural Research Center, Fargo, ND, USA.
¹⁵ John Innes Centre, Norwich Research Park, Norwich, UK. brande.wulff@jic.ac.uk.

PMID: 30718880
DOI: 10.1038/s41587-018-0007-9

Abstract

Disease resistance (R) genes from wild relatives could be used to engineer broad-spectrum resistance in domesticated crops. We combined association genetics with R gene enrichment sequencing (AgRenSeq) to exploit pan-genome variation in wild diploid wheat and rapidly clone four stem rust resistance genes. AgRenSeq enables R gene cloning in any crop that has a diverse germplasm panel.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromosome Mapping
Cloning, Molecular*
Crops, Agricultural / genetics*
Disease Resistance / genetics*
Genes, Plant*
Genetic Association Studies
Genetic Variation
Genomics
Genotype
Models, Genetic
Phenotype
Phylogeny
Plant Diseases / genetics*
Polymorphism, Single Nucleotide
Seedlings
Triticum / genetics

Abstract

Publication types

MeSH terms

Grants and funding